Fig. 5
Histone acetylation associated with p300 peaks in early myoblast differentiation. A C2C12 myoblasts were differentiated for 24 h (DM) and subjected to ChIP-seq with proliferating myoblasts (GM) as control. Shown are the profiles of average ChIP-seq read signals for the indicated histone acetylation marks at the sites of p300 occupancy. B The p300 sites in differentiating myoblasts were used to categorize enriched histone acetylation signals into 5 clusters with k-means clustering. The signal density is presented ± 1 kb from the peak canter. C Quantification of p300 enrichment signals was plotted as log2-fold change in differentiating myoblasts relative to proliferating myoblasts. D Gene expression levels from the RNA-seq are presented as FPKM (Fragments Per Kilobase of transcript per Million mapped reads) for genes associated to the indicated p300 cluster. E The p300 sites in each cluster were classified into distinct chromatin states. F Genome browser snapshot displays the read density of p300 and histone acetylation at the Tbc1d1 locus. Blue bars show the Refseq gene position and the colours of ChromHMM track below correspond to that assigned to each chromatin state