Fig. 2

Quality control of the RNA-seq datasets. A distribution of base quality scores is shown for two biological replicates of RNA-seq of C2C12 myoblasts following 24 h of differentiation (DM1, DM2) with proliferating myoblasts as controls (GM1, GM2). B The distribution of GC content across the reads. C The percentage of unique and duplicate reads from all mapped reads in each dataset. D The distribution of read coverage across the gene body. E The alignment of reads to known gene features in the reference genome (CDS, coding sequence; TSS, transcription start site; TES, transcription end site). F Heatmap of Spearman correlation coefficients calculated between log₂- transformed, nonzero FPKM expression values in both replicates of proliferating and differentiating myoblasts