Fig. 9

The role of immune subtypes in anti-PD-1 treatment cohort. Heatmaps of 28 previously reported immune cells infiltration among GBM immune subtypes in the pre-anti-PD-1 treatment group (a) and post-anti-PD-1 treatment group (b). c Distribution of immune subtypes among responders and non-responders in pre-anti-PD-1treatment samples. d Changes of immune subtypes in paired pre- and post-anti-PD-1 treatment samples. e Kaplan–Meier curve showing OS analysis of immune subtypes (IS1-IS3) in pre-anti-PD-1treatment samples. IS4 had only one sample and therefore was not included in this analysis. f Kaplan–Meier curve showing progression-free survival analysis of immune subtypes in pre-anti-PD-1treatment samples. IS4 had only one sample and therefore was not included in this analysis. Volcano plots showing DEGs between responders and non-responders in pre- (g) and post-anti-PD-1 treatment (i) samples. Red represented DEGs that were upregulated by responders relative to non-responders, while green represented DEGs that were downregulated. GO biological process enrichment analysis of DEGs between responders and non-responders in pre- (h) and post-anti-PD-1 treatment (j) samples